Introduction
Description: Modified Chu/Gamborg basal medium. Contains the macronutrients described by Chu (1975) & the micronutrients described by Gamborg, et al. (1968).
Solubility |
Water |
Physical Form |
Solid |
Storage Temp. |
2-6 °C |
Grams of powder to prepare 1 Liter |
4.1 |
Other Notes |
Plant Tissue Culture Tested |
UPC / SKU |
N492 |
Storage Temp. |
2-6 °C |
Tariff Code |
3822.00.5090 |
Risk Info (R) |
8-36/37/38 |
Safety Info (S) |
17-26-36 |
No information available
PROD. # |
COMPONENT NAME |
mg/L |
A305 |
Ammonium Sulfate |
463 |
B210 |
Boric Acid |
3.0 |
C266 |
Calcium Chloride, Anhydrous |
125.33 |
C350 |
Cobalt Chloride Hexahydrate |
0.025 |
C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
E410 |
EDTA, Disodium Salt |
37.26 |
F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
M150 |
Magnesium Sulfate, Anhydrous |
90.37 |
M250 |
Manganese Sulfate |
10 |
P840 |
Potassium Iodide |
0.75 |
P100 |
Potassium Nitrate |
2830 |
P846 |
Potassium Phosphate, Monobasic, Anhydrous |
400 |
M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
Z101 |
Zinc Sulfate, Heptahydrate |
2.0 |
I703 |
myo-Inositol |
100 |
N765 |
Nicotinic Acid |
1.0 |
P866 |
Pyridoxine HCl |
1.0 |
T390 |
Thiamine HCl |
10 |
N492 NB Basal Medium Modified Chu/ Gamborg Basal Medium
Synonym: WPM Basal Salts
Properties |
Form: |
Powder |
Appearance: |
White to Yellow Powder |
Application: |
Plant Tissue Culture |
Solubility: |
Water |
Typical Working Concentration: |
4.10g/L |
Storage Temp: |
2-6°C |
Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5 |
Formula (mg/L) |
Ammonium Sulfate |
463 |
Boric Acid |
3 |
Calcium Chloride, Anhydrous |
125.33 |
Cobalt Chloride.6H2O |
0.025 |
Cupric Sulfate.5H2O |
0.025 |
Na2 EDTA.2H2O |
37.26 |
Ferrous Sulfate.7H2O |
27.8 |
Magnesium Sulfate, Anhydrous |
90.37 |
Manganese Sulfate.H2O |
10 |
Molybdic Acid (Sodium Salt).2H2O |
0.25 |
Potassium Iodide |
0.75 |
Potassium Nitrate |
2830 |
Potassium Phosphate, Monobasic |
400 |
Zinc Sulfate.7H2O |
2 |
myo-Inositol |
100 |
Nicotinic Acid (Free Acid) |
1 |
Pyridoxine.HCl |
1 |
Thiamine.HCl |
10 |
Application Notes
Plant Tissue Culture Tested
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659- 668.
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.