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NLN Basal Medium
Product ID: N479

Introduction

Description: Contains the macro- and micronutrients, and organic constituents as described by Lichter (1982). Without sucrose.
This medium was derived from that described by Nitsch & Nitsch and was developed to support the initiation and growth of haploid plants from anther and pollen cultures of Brassica napus. This medium contains the equivalent of 500 mg/L Calcium Nitrate tetrahydrate as described in the original formulation.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 1.77
Other Notes Plant Tissue Culture Tested
UPC / SKU N479
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
B210 Boric Acid 10
C180 Calcium Nitrate 345
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E676 Ferric Sodium EDTA 36.7
M150 Magnesium Sulfate, Anhydrous 61
M250 Manganese Sulfate 18.95
P100 Potassium Nitrate 125
P846 Potassium Phosphate, Monobasic, Anhydrous 125
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 10
B140 D-Biotin 0.05
F430 Folic Acid 0.5
G229 L-Glutamine 800
G394 Glutathione (Reduced), Sodium Salt 30
G503 Glycine 2.0
I703 myo-Inositol 100
N765 Nicotinic Acid 5
P866 Pyridoxine HCl 0.5
S807 L-Serine 100
T390 Thiamine HCl 0.5

N479 NLN Basal Medium

Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 1.77g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and organic constituents as described by Lichter (1982).
Formula (mg/L)
Boric Acid 10
Calcium Nitrate (dried)  347
Cobalt Chloride.6H2O  0.025
Cupric Sulfate.5H2O  0.025
Ferric Sodium EDTA  36.7
Magnesium Sulfate, Anhydrous  61
Manganese Sulfate.H2O  18.95
Molybdic Acid (Sodium Salt).2H2O 0.25
Potassium Nitrate 125
Potassium Phosphate, Monobasic  125
Zinc Sulfate.7H2O  10
D-Biotin  0.05
Folic Acid 0.5
L-Glutamine 800
Glutathione 30
Glycine (Free Base)  2
myo-Inositol  100
Nicotinic Acid (Free Acid)  5
Pyridoxine.HCl 0.5
L-Serine 100
Thiamine.HCl  0.5

Application Notes
Plant Tissue Culture Tested Plant species: Brassica napus This medium was derived from that described by Nitsch & Nitsch and was developed to support the initiation and growth of haploid plants from anther and pollen cultures of Brassica napus. This medium contains the equivalent of 500 mg/L calcium nitrate tetrahydrate as described in the original formulation.

References
Lichter, R. 1981. Z. Pflanzenphysiol. 103: 119. Lichter, R. 1982. Z. Pflanzenphysiol. 105: 427