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TheLuc-Pair™ miRLuciferaseAssayKitprovides an efficientsystem in a convenient 96-wellplateformat formeasuringfireflyand Renilla luciferases sequentially.OptimizedforusewithGeneCopoeia miRNA3’ UTRTargetSequenceExpressionClones, Luc-PairmiRassays,enableseasy,costeffective validation of miRNAactivity.Luc-PairmiRLuciferase Assaysprovide the followingadvantages:
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- High sensitivity in a large dynamic range
- Dual reporter system
- Reproducible
- Cost effectiveness
- Simple assay format
- Suitable for HTS
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Figure1.The inhibitory effect of miRNA on a target sequence(3’ UTR) expression clonecan be measured with GeneCopoeia Luc-PairmiR Luciferase Assay kit. HEK 293 cellswereplated on a 6-wellplate. On the secondday,thecellsweretransfectedwith1.0 mg of targetsequence(3’ UTR)expressionclone(pEZX-MT01-Lin28 UTR-fLuc) and 1.4 mg of miRNA expressionvector(ormiRNAcontrolvector) as indicated in thefigure. Thecellsweretransferred to a 96-wellplate 18 hoursaftertransfection and culturedforanother 24 hours.Bothfireflyluciferase and Renilla luciferaseactivitiesweremeasured as describedanddatawasrecorded on Victor II machine.Fireflyluciferaseactivitywasthennormalizedwith Renilla luciferaseactivities in thesamewell.
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Figure2. Linear relationship between emitted light and amount of luciferase expression vector transfected in HEK293. HEK293cellswere plated on a 6-wellplate. On thesecondday,thecellsweretransfected withdifferentamounts of GeneCopoeiapMRO or pEZX-MT01 miRNA3” UTRTargetSequenceClone.Thecellsweretransferred to a 96-wellplate 18 hoursaftertransfection and culturedforanother 24 hours.Firefly luciferaseassaywasthenperformed on pMROvector(Fig.2a) and Renilla luciferaseassaywasperformed on pEZX-MT01 vector(Fig.2b) as described. |
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Catalog No. |
Name |
Size |
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LPFR-M010 |
Luc-Pair™ miRLuciferase Assay Kit (1) |
100 reactions |
LPFR-M100 |
Luc-Pair™ miRLuciferase Assay Kit (10) |
1000 reactions |
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Advantages |
- Ideal for GeneCopoeia miRNA 3’ UTR Target Sequence Expression Clones
- Easy to use — less hands-on time and quick results
- Suitable for micro-plate format — save time and money
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