Introduction
Description: Contains the Woody plant medium macro- and micronutrients as described by Lloyd & McCown (1981).
This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium Nitrate has been eliminated from this medium and replaced with Potassium Sulfate.
Solubility |
Water |
Physical Form |
Solid |
Storage Temp. |
2-6 °C |
Grams of powder to prepare 1 Liter |
2.3 |
Other Notes |
Plant Tissue Culture Tested |
UPC / SKU |
L154 |
Storage Temp. |
2-6 °C |
Tariff Code |
3822.00.5090 |
Risk Info (R) |
8-36/37/38 |
Safety Info (S) |
17-26-36 |
No information available
PROD. # |
COMPONENT NAME |
mg/L |
A300 |
Ammonium Nitrate |
400 |
B210 |
Boric Acid |
6.2 |
C266 |
Calcium Chloride, Anhydrous |
72.5 |
C180 |
Calcium Nitrate |
386 |
C375 |
Cupric Sulfate, Pentahydrate |
0.25 |
E410 |
EDTA, Disodium Salt |
37.3 |
F263 |
Ferrous Sulfate, Heptahydrate |
27.85 |
M150 |
Magnesium Sulfate, Anhydrous |
180.7 |
M250 |
Manganese Sulfate |
22.3 |
P846 |
Potassium Phosphate, Monobasic, Anhydrous |
170 |
P854 |
Potassium Sulfate, Anhydrous |
990 |
M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
L154 Lloyd & McCown Woody Plant Basal Salt Mixture
Synonym: WPM Basal Salts
Properties |
Form: |
Powder |
Appearance: |
White to Yellow Powder |
Application: |
Plant Tissue Culture |
Solubility: |
Water |
Typical Working Concentration: |
2.30g/L |
Storage Temp: |
2-6°C |
Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5 |
Formula (mg/L) |
Ammonium Nitrate |
400 |
Boric Acid |
6.2 |
Calcium Chloride, Anhydrous |
72.5 |
Calcium Nitrate |
386 |
Cupric Sulfate.5H2O |
0.25 |
Na2 EDTA.2H2O |
37.3 |
Ferrous Sulfate.7H2O |
27.85 |
Magnesium Sulfate, Anhydrous |
180.7 |
Manganese Sulfate.H2O |
22.3 |
Molybdic Acid (Sodium Salt).2H2O |
0.25 |
Potassium Phosphate, Monobasic |
170 |
Potassium Sulfate |
990 |
Zinc Sulfate.7H2O |
8.6 |
Application Notes
Plant Tissue Culture Tested
Plant species: Kalmia latifolia (Mountain Laurel), Rhododendron spp. This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium nitrate has been eliminated from this medium and replaced with potassium sulfate. The levels of ammonium nitrate are ¼x compared to MS. Nitrogen is also provided by calcium nitrate.
References
Lloyd, G and BH McCown. 1981. Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture. Proc. Int. Plant Prop. Soc. 30:421-427.