Introduction
Description:Contains the macro-, micronutrients, and vitamins as described by Driver & Kuniyuki (1984) and McGranahan, et al (1987).
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 μM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
5.32 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
D2470 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
1416 |
| B210 |
Boric Acid |
4.8 |
| C266 |
Calcium Chloride, Anhydrous |
112.5 |
| C180 |
Calcium Nitrate |
1367 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.25 |
| E410 |
EDTA, Disodium Salt |
45.4 |
| F263 |
Ferrous Sulfate, Heptahydrate |
33.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
361.49 |
| M250 |
Manganese Sulfate |
33.5 |
| N458 |
Nickel Sulfate Hexahydrate |
0.005 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
265 |
| P854 |
Potassium Sulfate, Anhydrous |
1559 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.39 |
| Z897 |
Zinc Nitrate, Hexahydrate |
17 |
| G503 |
Glycine |
2.0 |
| I703 |
myo-Inositol |
100 |
| N765 |
Nicotinic Acid |
1.0 |
| T390 |
Thiamine HCl |
2.0 |
D2470
DKW Basal Medium with Vitamins
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
5.32 g/L |
| Storage Temp: |
2 - 6° C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Driver and Kuniyuki (1984) and corrected by McGranahan, et al. (1987).
pH = 3.5 - 4.5 |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
1416 |
| Boric Acid |
4.8 |
| Calcium Chloride, Anhydrous |
112.5 |
| Calcium Nitrate |
1367 |
| Cupric Sulfate·5H2O |
0.25 |
| Na2 EDTA·2H2O |
45.4 |
| Ferrous Sulfate·7H2O |
33.8 |
| Magnesium Sulfate, Anhydrous |
361.49 |
| Manganese Sulfate·H2O |
33.5 |
| Molybdic Acid (Sodium Salt)·2H2O |
0.39 |
| Nickel Sulfate·6H2O |
0.005 |
| Potassium Phosphate, Monobasic |
265 |
| Potassium Sulfate |
1559 |
| Zinc Nitrate·6H2O |
17 |
| Myo-Inositol |
100 |
| Glycine |
2 |
| Nicotinic Acid |
1 |
| Thiamine Hydrochloride |
2 |
Application Notes
Plant species: Northern California Walnut (Juglans hindsii)
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 µM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.
References
Driver, J.A. and A.H. Kuniyuki. 1984. In vitro propagation of Paradox walnut rootstock.
HortScience 19:507-509.
McGranahan, GH, et al. 1962. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.
