CF™Dyes The next-generation dyes for protein labeling

CF dyes are a series of fluorescent dyes with superior brightness and photostability. Primarily designed for labeling antibodies, many of our CF dyes (near IR CF dyes in particular) outperform the Alexa Fluor dyes (For more detailed comparison, please download CF dye flyer and Near IR CF dye flyer . CF dye antibody conjugates generally give better signal to noise ratio than Alexa Fluor antibody conjugates because of the fewer charges CF dyes carry. CF dye products are currently offered in three categories: CF dye-labeled secondary antibodies Table A; other CF dye-labeled products Table B; and reactive CF dyes and labeling kits (Table C). More CF dye products will be offered in the near future as they become available. We also do custom conjugations if you have a primary antibody or any particular substrate molecule you would like us to label with one of the CF dyes.

Figure 1. HeLa cells were stained with CF 555 phalloidin (red), mouse anti-a-tubulin followed by goat anti-mouse CF 488A IgG (green) and DAPI (blue), respectively.

For more information on each product, click the product name in the table. To license CF dye technology, please contact customerservice@atzlabs.com">customerservice@atzlabs.com.

Fluorescence Emission Spectra of CF Dyes

Table A. CF Dye-labeled Secondary Antibody Conjugates

Cat#	Product Name	*Abs/Em (nm)**	Unit Size
20010	CF488A goat anti-mouse IgG (H+L), at 2 mg/mL	490/515	0.5 mL
20011	CF 488A F(ab')2 fragment of goat anti-mouse IgG(H+L), 2 mg/mL	490/515	0.25 mL
20012	CF 488A goat anti-rabbit IgG(H+L), 2 mg/mL	490/515	0.5 mL
20013	CF 488A F(ab')2 fragment of goat anti-rabbit IgG(H+L), 2 mg/mL	490/515	0.25 mL
20030	CF555 goat anti-mouse IgG (H+L), at 2 mg/mL	555/565	0.5 mL
20032	CF 555 F(ab')2 fragment of goat anti-mouse IgG(H+L), 2 mg/mL	555/565	0.25 mL
20033	CF 555 goat anti-rabbit IgG(H+L), 2 mg/mL	555/565	0.5 mL
20035	CF 555 F(ab')2 fragment of goat anti-rabbit IgG(H+L), 2 mg/mL	555/565	0.25 mL
20036	CF 555 goat anti-guinea pig IgG(H+L), 2 mg/mL	555/565	0.5 mL
20040	CF 647 goat anti-mouse IgG(H+L), 2 mg/mL	650/665	0.5 mL
20042	CF 647 F(ab')2 fragment of goat anti-mouse IgG(H+L), 2 mg/mL	650/665	0.25 mL
20043	CF 647 goat anti-rabbit IgG(H+L), 2 mg/mL	650/665	0.5 mL
20045	CF 647 F(ab')2 fragment of goat anti-rabbit IgG(H+L), 2 mg/mL	650/665	0.25 mL
20050	CF 660 goat anti-mouse IgG(H+L), 2 mg/mL	667/685	0.5 mL
20053	CF 660 goat anti-rabbit IgG(H+L), 2 mg/mL	667/685	0.5 mL
20065	CF680 goat anti-mouse IgG(H+L), highly cross-absorbed, 2 mg/mL	681/698	0.25 mL
20067	CF680 goat anti-rabbit IgG(H+L), highly cross-absorbed, 2 mg/mL	681/698	0.25 mL
20070	CF 750 goat anti-mouse IgG(H+L), 2 mg/mL	755/777	0.5 mL
20073	CF 750 goat anti-rabbit IgG(H+L), 2 mg/mL	755/777	0.5 mL
20077	CF770 goat anti-mouse IgG(H+L), highly cross-absorbed, 2 mg/mL	770/797	0.25 mL
20078	CF770 goat anti-rabbit IgG(H+L), highly cross-absorbed, 2 mg/mL	770/797	0.25 mL

Figure 2. HeLa cells were stained with mouse anti-golgin 97 and rabbit anti-COX IV primary antibodies followed by CF488A goat anti-mouse and CF555 goat anti-rabbit secondary antibodies. CF647-phalloidin was used to label F-actin and Hoechst 33342 was used to stain for DNA. (Green=golgi, red=mitochondria, blue=nucleus, and magenta=cytoskeleton).

Figure 3. Rat hippocampal neurons stained with SynaptoGreen C4 (also called FM1-43, cat# 70020) and CF647 hydrazide (cat# 70020). SynaptoGreen C4 stains synaptic vesicles (green) while CF647 hydrazide (cat# 92136) stains the cell bodies (red).

Cat#	Product Name	Abs/Em (nm)	Application	Unit Size
29005	Annexin V, CF488 conjugate	490/515	Apoptosis	0.5 mL
29004	Annexin V, CF555 conjugate	555/565	Apoptosis	0.5 mL
29003	Annexin V, CF647 conjugate	650/665	Apoptosis	0.5 mL
00034	Phalloidin, CF405 conjugate	405/460	Cell skeleton (actin)	300 U
00042	Phalloidin, CF488A conjugate	488/516	Cell skeleton (actin)	300 U
00040	Phalloidin, CF555 conjugate	555/565	Cell skeleton (actin)	300 U
00041	Phalloidin, CF647 conjugate	650/665	Cell skeleton (actin)	300 U

Table C. Reactive CF Dyes and Labeling Kits

Cat#	Product Name	*Abs/Em (nm)**	Function	Unit Size
92120	CF488 succinimidyl ester	488/516	Amine-reactive	1 umole
92130	CF555 succinimidyl ester	552/574	Amine-reactive	1 umole
92135	CF647 succinimidyl ester	650/674	Amine-reactive	1 umole
92136	CF647 hydrazide	650/674	Aldehyde- or ketone-reactive	1 mg
92137	CF660 succinimidyl ester	664/685	Amine-reactive	1 umole
92139	CF680 succinimidyl ester	679/701	Amine-reactive	1 umole
92142	CF750 succinimidyl ester	752/786	Amine-reactive	1 umole
92150	CF770 succinimidyl ester	766/800	Amine-reactive	1 umole
92160	VivoBrite rapid antibody labeling kit for in vivo imaging, CF680 SE	679/701	Amine-reactive	3 labelings
92161	VivoBrite rapid antibody labeling kit for in vivo imaging, CF750 SE	752/786	Amine-reactive	3 labelings
92162	VivoBrite rapid antibody labeling kit for in vivo imaging, CF770 SE	766/800	Amine-reactive	3 labelings