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Chu's N6 Basal Medium with Vitamins
Product ID: C167

Introduction

Description: Contains the macro- and micronutrients, and vitamins as described by Chu (1975).
Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus from rice pollen cultures. Ammonium Nitrate has been replaced by Ammonium Sulfate. The molar concentration of NH4 is 7.0 mM compared to 20.6mM for MS.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 3.99
Other Notes Plant Tissue Culture Tested
UPC / SKU C167
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A305 Ammonium Sulfate 463
B210 Boric Acid 1.6
C266 Calcium Chloride, Anhydrous 125.33
E410 EDTA, Disodium Salt 37.25
F263 Ferrous Sulfate, Heptahydrate 27.85
M150 Magnesium Sulfate, Anhydrous 90.37
M250 Manganese Sulfate 3.3
P840 Potassium Iodide 0.8
P100 Potassium Nitrate 2830
P846 Potassium Phosphate, Monobasic, Anhydrous 400
Z101 Zinc Sulfate, Heptahydrate 1.5
G503 Glycine 2.0
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
T390 Thiamine HCl 1.0

C167 Chu N6 Basal Medium w/ Vitamins

Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 3.99g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Chu et al. (1975). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Sulfate  463
Boric Acid 1.6
Calcium Chloride, Anhydrous  125.33
Na2 EDTA·2H2O 37.25
Ferrous Sulfate·7H2O 27.85
Magnesium Sulfate, Anhydrous 90.37
Manganese Sulfate·H2O  3.3
Potassium Iodide 0.8
Potassium Nitrate 2830
Potassium Phosphate, Monobasic 400
Zinc Sulfate·7H2O 1.5
Glycine (Free Base) 2
Nicotinic Acid (Free Acid) 0.5
Pyridoxine·HCl 0.5
Thiamine·HCl 1

Application Notes
Plant Tissue Culture Tested
Plant species: rice (Oryza sativa) Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus from rice pollen cultures.
Ammonium nitrate has been replaced by ammonium sulfate. The molar concentration of NH4 + is 7.0 mM compared to 20.6mM for MS

References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659- 668.