Break Through in RNA Isolation
One-Step Method without Phase Separation |
Product Description
RNAzolŪRT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patent-pending reagent provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzolŪRT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment. RNAzolŪRT separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. Supplementing RNAzolŪRT with water makes DNA, proteins and polysaccharides insoluble. |
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A biological sample is homogenized or lysed in RNAzol®RT. DNA, proteins, polysaccharides and other molecules are precipitated from the homogenate/lysate by the addition of water and removed by centrifugation. The pure RNA is isolated from the resulting supernatant by ethanol precipitation, followed by washing and solubilization.
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- The isolation procedure can be completed in less than one hour. The isolated RNA is ready for use in RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
- RNAzol®RT isolates total RNA, with mRNA and small RNA as separate fractions. Small RNA contains RNA <200 bases, including 10 - 15 base miRNA.
- In addition, RNAzol®RT allows for the simultaneous isolation of RNA and DNA.
- The RNAzol®RT procedure is performed at room temperature, including centrifugation.
- Due to lack of co-isolation of impurities, the RNAzol®RT procedure yields RNA pellets that are significantly
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STABILITY/STORAGE: RNAzol®RT is stable when stored at room temperature for at least two years from date of purchase. |
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