Store at -20°C Shelf life: 2 years Description Tiger
XTaq mix (x2) is
a premixed, ready-to-use solution containing XTaq DNA Polymerase, dNTPs, Mg2+
and Reaction Buffer at optimal concentrations for efficient amplification of
DNA templates by PCR. To prepare the final PCR, only primers and template DNA
are added. XTaq Mix contributes to highly reproducible PCR by reducing
the risk of pipetting errors, miscalculation and contamination. It also
contributes to higher sensitivity by adding enhancer. XTaq is a mixture of Taq
and High Fidelity polymerase, therefore, this specially formulated XTaq plus
allow amplification of the higer fidelity and longer templates than the
single-enzyme formulations. It is also a better choice for amplifying complex
template, such as GC-rich template. And it is suitable as a direct replacement
for ordinary Taq Polymerase in most applications. In addition, Using XTaq
results in 3´-dA overhangs PCR products, which can be used in TA clone.
Applications
Composition of Tiger XTaq Mix 0.4U/ul
XTaq DNA polymerase, 2x PCR Buffer, 0.4mM dNTPs, 4mM MgSO4, 0.02%
bromophenol blue. XTaq mix Buffer is a proprietary formulation
optimized for robust performance in PCR. |
