Concentration: 5U/μl
Store at -20°C Description Taq
500U + dNTPs is a thermostable recombinant DNA polymerase derived from
thermophilic bacterium Thermus aquaticus. Its molecular weight is 94
kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple
template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has
5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity
that results in a 3'-dA overhangs PCR product.
Unit Definition One
unit is defined as the amount of the enzyme required to catalyze the
incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at
70°C using hering sperm DNA as substrate.
Storage Buffer 20mM TrisCl ( pH8.0), 100mM KCl, 3.2mM MgCl2, 1mM DTT, 0.1% Triton X-100, 0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol.
10X PCR Buffer with Mg2+ 100mM Tris-HCl (PH 8.8), 500mMKCl, 1%Triton-X-100, 16mM MgCl2.
Applications
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