Store at -20°C Shelf life: 2 years Description PCR
Ready Mix (2x) is a premixed, ready-to-use solution containing Taq DNA
Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations
for efficient amplification of DNA templates by PCR. To prepare the
final PCR, only primers and template DNA are added. This pre-mixed
formulation saves time and reduces contamination due to the fewer
pipetting steps required for PCR set up. The mix retains all features
of Taq DNA Polymerase. Taq DNA Polymerase is a thermostable recombinant
DNA polymerase derived from thermophilic bacterium Thermus aquaticus.
Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA
target up to 5 kb (simple template). The elongation velocity is
0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks
of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR
product.
Features
Applications
Taq DNA polymerase is supplied in 2X Taq buffer, dNTPs, 3mM MgSO4 and bromophenol blue. Taq Ready mix buffer is a proprietary formulation optimized for robust performance in PCR. Perform PCR using the following thermal cycling conditions:
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