UltraClean® Plant RNA Isolation Kit

Description


The UltraCleanŽ Plant RNA Isolation Kit is designed to isolate total RNA from a wide variety of plant materials. Sample sizes from 5 to 100 mg can be processed. All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications.

The UltraCleanŽ Plant RNA Isolation Kit is designed to isolate total RNA from a wide variety of plant materials. Sample sizes from 5 to 100 mg can be processed. All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications.

 
 

Features
  • Rapid isolation of total RNA from plant tissues in only 35 minutes
  • Yields up to 60 μg high quality RNA for RT and RT-PCR
  • Safe and easy protocol uses NO phenol/chloroform extraction, CsCl gradients or ethanol precipitation
  • Includes all ready-to
  • use RNase-free reagents and all required spin filters and tubes

Storage Conditions
Store at room temperature for up to 2 years

Specifications
Format Silica Spin Filter Tubes
Method Manual Homogenization
Starting Amount 5-25mg
Binding Capacity Up to 60μg per filter
Throughput 1 - 24 samples
Time 35 minutes
 
Plant Types Tested with the UltraClean® Plant RNA Kit
Spinach Mint Strawberry Lime
Radish Alfalfa Wheat Bean sprout
Corn Tomato Rice Bell pepper
Grape Leaf Grass Cowpea Arabidopsis
 

Research and Development
Total RNA From Plantst
 
Lane 1: Marker Lane 4: Cauliflower Lane 7: Corn
Lane 2: Spinach Lane 5: Broccoli Lane 8: Sunflower
Lane 3: Onion Lane 6: Radish Lane 9: Wheat Grass

Kit Components
Component 13300-20 13300-50
Solution PMR1 1 x 25 ml 1 x 60 ml
Solution PMR2 1 x 12 ml 1 x 30 ml
Solution PMR3 1 x 8 ml 1 x 15 ml
Solution PMR4 1 x 20 ml 2 x 25 ml
Solution PMR5 1 x 15 ml 1 x 30 ml
Solution PMR6 1 x 2 ml 1 x 5 ml
Spin Filters in Tubes 20 50
Collection Tubes 40 100