Description
The UltraCleanŽ Plant RNA Isolation Kit is designed to isolate total RNA from a wide variety of plant materials. Sample sizes from 5 to 100 mg can be processed. All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications.
The UltraCleanŽ Plant RNA Isolation Kit is designed to isolate total RNA from a wide variety of plant materials. Sample sizes from 5 to 100 mg can be processed. All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications. |
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Features |
- Rapid isolation of total RNA from plant tissues in only 35 minutes
- Yields up to 60 μg high quality RNA for RT and RT-PCR
- Safe and easy protocol uses NO phenol/chloroform extraction, CsCl gradients or ethanol precipitation
- Includes all ready-to
- use RNase-free reagents and all required spin filters and tubes
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Storage Conditions |
Store at room temperature for up to 2 years
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Specifications |
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Format |
Silica Spin Filter Tubes |
Method |
Manual Homogenization |
Starting Amount |
5-25mg |
Binding Capacity |
Up to 60μg per filter |
Throughput |
1 - 24 samples |
Time |
35 minutes |
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Plant Types Tested with the UltraClean® Plant RNA Kit |
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Spinach |
Mint |
Strawberry |
Lime |
Radish |
Alfalfa |
Wheat |
Bean sprout |
Corn |
Tomato |
Rice |
Bell pepper |
Grape Leaf |
Grass |
Cowpea |
Arabidopsis |
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Research and Development
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Total RNA From Plantst
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Lane 1: Marker |
Lane 4: Cauliflower |
Lane 7: Corn |
Lane 2: Spinach |
Lane 5: Broccoli |
Lane 8: Sunflower |
Lane 3: Onion |
Lane 6: Radish |
Lane 9: Wheat Grass |
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Kit Components |
Component |
13300-20 |
13300-50 |
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Solution PMR1 |
1 x 25 ml |
1 x 60 ml |
Solution PMR2 |
1 x 12 ml |
1 x 30 ml |
Solution PMR3 |
1 x 8 ml |
1 x 15 ml |
Solution PMR4 |
1 x 20 ml |
2 x 25 ml |
Solution PMR5 |
1 x 15 ml |
1 x 30 ml |
Solution PMR6 |
1 x 2 ml |
1 x 5 ml |
Spin Filters in Tubes |
20 |
50 |
Collection Tubes |
40 |
100 |
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