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Schenk & Hildebrandt Modified Basal Medium w/ Sucrose, w/o Vitamins
Product ID: S811

Introduction

Description: Contains the ½ macro- and ½ micronutrients and ½ the vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 13.2
Other Notes Plant Tissue Culture Tested
UPC / SKU S811
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A113 Ammonium Phosphate 300
B210 Boric Acid 5
C266 Calcium Chloride, Anhydrous 151
C350 Cobalt Chloride Hexahydrate 0.1
C375 Cupric Sulfate, Pentahydrate 0.2
E410 EDTA, Disodium Salt 20
F263 Ferrous Sulfate, Heptahydrate 15
M150 Magnesium Sulfate, Anhydrous 195.4
M250 Manganese Sulfate 10
P840 Potassium Iodide 1
P100 Potassium Nitrate 2500
M651 Sodium Molybdate (VI), Dihydrate 0.1
Z101 Zinc Sulfate, Heptahydrate 1
S391 D-Sucrose 10,000

S811 Schenk & Hildebrandt Modified Basal Medium Contains 10 g/L Sucrose

Synonym: Schenk & Hildebrandt Basal Salts w/ Sucrose

Properties
Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 13.20g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Schenk and Hildebrandt (1972) with the modification of added Sucrose at 10 g/L.
Formula (mg/L)
Ammonium Phosphate, Monobasic  300
Boric Acid 5
Calcium Chloride, Anhydrous 151
Cobalt Chloride.6H2O 0.1
Cupric Sulfate.5H2O 0.2
Na2 EDTA.2H2O 20
Ferrous Sulfate.7H2O  15
Magnesium Sulfate, Anhydrous 195.4
Manganese Sulfate.H2O  10
Molybdic Acid (Sodium Salt).2H2O  0.1
Potassium Iodide  1
Potassium Nitrate 2500
Zinc Sulfate.7H2O 1
Sucrose  10,000

Application Notes
Plant Tissue Culture Tested

References
Schenk, RU & AC Hildebrandt. 1972. Medium and Techniques for Induction and Growth of Monocotyledonous and Dicotyledonous Plant Cell Cultures. Can. J. Bot. 50: 199-204.