Biotinylation, HRP and FITC Labeling Kits

Biotinylation, HRP and FITC Protein/Antibody Labeling Kits

Cat # 80220 Horseradish peroxidase (HRP) conjugation kit for antigen/antibodies Contains pre-activated HRP, Simply mix antibodies with HRP for conjugation.
Cat # 80230 Antibody/Protein conjugation to FITC. Labeling in 1-2 hrs for 2 reactions.
Cat # 80300 Protein (antigen/antibodies) Biotinylation Kit. Biotinylation in 1-2 hrs for 2 reactions.

Pre-Activated BSA, KLH for Peptide/Protein Conjugation

Cat # 80240 Peptide/Protein conjugation to Pre-activated BSA Kit (simply mix Peptide/proteins with BSA, for 2 conj.)
Cat # 80250 Peptide/Protein conjugation to Pre-activated KLH Kit (simply mix Peptide/proteins with KLH, for 2 conj)

Pre-Activated Agarose for Coupling Peptides, Proteins or Antibodies

Cat # 110100-CG Preactivated Cys-Link-Affinity Gel Sepharose (for Cys-containing peptides/proteins) Contains pre-activated gel, Simply mix Peptide/proteins for conjugation.
Cat# 110200-NG Preactivated N-Link-Affinity Gel Agarose (for NH2-containing peptides/proteins) Contains pre-activated gel, Simply mix Peptide/proteins for conjugation.

Western Blot Recycling Kit

Re-use Old or New Western Blots using ADI's Western Blot Recycling Kit

Strip antibodies at RT; No heating required. No mercaptoethanol; No pungent smell.
Just soak blots in stripping soln for 10-15 min. Blots are reusable within 15-60 min.
Multiple stripping; reuse blots 5-10 times Blots can be probed with the same or different antibodies.

Western Blot Recycling Kit Cat # 90100 Sufficient for 30-50 mini-blots List of publications (please see update list at the web site)

Humphreys 2002, Endocrinology 143, 3641| Used Nitrocellulose| Stripped 4 times and reprobed with other antibodies Dwivedi 2002, JBC 277, 29643, Used Nitrocellulose, Stripped phosphoantibodies and reprobed with other antibodies McClelland 2002, J Appl Physiol, 2002| 92: 1573, Used PVDF, stripped and reprobed with various MCTs antibodies Jackson 2001 , J Immunol 166, 952-958| Used Nitrocellulose| primary antibody stripped and reprobed with beta-actin

Annotate or Mark Western Blot Membranes with an Antigen Pen ™

Using a proprietary and patented technology, ADI has designed and developed specialized fountain pens, called Antigen-Antibody pens��, that transfer the antigens or antibodies pre-formulated in the ink. The antigen pens can be used to write or mark the blotting membranes in any form or shape or size. One can mark the size of the markers (e.g., 15-150 kDa) or the lanes (1-7). The secondary antibody conjugate reacts with the primary antibodies (mouse, rabbit etc.) being used for the Western and also the antibody (pre-mixed in the in the antigen pens) and the pen marking as well. The signal is produced and recorded from the protein bands, the antigen writing (lanes, mol wts) allowing immediate identification of samples and protein size. There is no need to compare the image with the original blot. In addition, Antigen-Antibody Pens are designed for animals commonly used for primary antibody production. Therefore, if one is using rabbit primary antibody and the antigen pens specific for rabbit, then the secondary antibody must be anti-rabbit for the writing or the protein bands to be visible. The usage of antigen pens provides a built-in positive control for the entire Western procedure.

Cat # Product Description Cat # Product Description
PEN13-SET Antigen-Antibody Pens, a set of any 3 pens PEN-M2 Antigen-Antibody Pen For Mouse antibodies
PEN-B9 Antigen-Antibody Pen For Biotin-tagged antibodies (all species) PEN-P6 Antigen-Antibody Pen For G. Pig antibodies
PEN-C5 Antigen-Antibody Pen For Chicken antibodies PEN-R1 Antigen-Antibody Pen For Rabbit antibodies
PEN-G3 Antigen-Antibody Pen For Goat antibodies PEN-S4 Antigen-Antibody Pen For Sheep antibodies
PEN-H7 Antigen-Antibody Pen For Human antibodies PEN-T8 Antigen-Antibody Pen For Hamster antibodies