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Life Technologies India
PhytoTechnology Laboratories

Orchid Maintenance Medium with Charcoal & Agar
Product ID: P658

Introduction

Description: This medium is a modification of P668 Orchid Maintenance/ Replate Medium. It was originally developed for the culture of Phalaenopsis stem props; it is now widely used as a seed sowing and replate medium for many epiphytic orchid species.

Solubility Water (Partially)
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 35.31
Other Notes Plant Tissue Culture Tested
UPC / SKU P658
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 825
B210 Boric Acid 3.1
C266 Calcium Chloride, Anhydrous 166
C350 Cobalt Chloride Hexahydrate 0.0125
C375 Cupric Sulfate, Pentahydrate 0.0125
E410 EDTA, Disodium Salt 37.3
F263 Ferrous Sulfate, Heptahydrate 27.85
M150 Magnesium Sulfate, Anhydrous 90.35
M250 Manganese Sulfate 8.45
P840 Potassium Iodide 0.415
P100 Potassium Nitrate 950
P846 Potassium Phosphate, Monobasic, Anhydrous 85
M651 Sodium Molybdate (VI), Dihydrate 0.125
Z101 Zinc Sulfate, Heptahydrate 5.3
C325 Activated Charcoal 2000
A111 Agar 8000
I703 myo-Inositol 100
M825 MES, Free Acid 1000
N765 Nicotinic Acid 1.0
P775 Peptone from Meat 2000
P866 Pyridoxine HCl 1.0
S391 D-Sucrose 20,000
T390 Thiamine HCl 10

P658 Orchid Maintenance Medium Contains Charcoal and Agar

Properties
Form: Powder
Appearance: Gray powde
Application: Orchid Cultur
Solubility: Partially Soluble in Wate
Typical Working Concentration: 35.31 g/L
Storage Temp: 2-6 C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: pH = 4.75 - 5.75
Formula (mg/L)
Ammonium Nitrate 825
Boric Acid 3.1
Calcium Chloride, Anhydrous 166
Cobalt Chloride.6H2O 0.0125
Cupric Sulfate.5H2O 0.0125
Na2EDTA.2H2O 37.3
Ferrous Sulfate.7H2O 27.85
Magnesium Sulfate, Anhydrous 90.35
Manganese Sulfate.H2O 8.45
Molybdic Acid (Sodium Salt) .2H2O 0.125
Potassium Iodide 0.415
Potassium Nitrate 950
Potassium Phosphate, Monobasic 85
Zinc Sulfate.7H2O 5.3
Activated Charcoal 2000
Agar 8000
MES (Free Acid) 1000
myo-Inositol 100
Nicotinic Acid (Free Acid) 1
Peptone from Meat 2000
Pyridoxine.HCl 1
Sucrose 20,000
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Many epiphytic orchid species
This medium is a modification of P668 Orchid Maintenance/ Replate Medium; P658 differs from P668 in that it contains 8.0 g/L of agar. It was originally develo ped for the culture of Phalaenopsis stem props; it is now widely used as a seed sowing and replate medium for many epiphytic orchid species