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Life Technologies India
PhytoTechnology Laboratories

Orchid Maintenance/Replate Medium without Charcoal & Agar
Product ID: O139

Introduction

Description: This medium is a modification of our original orchid replate medium (P668) except without charcoal (and agar). This medium permits the culture of orchids in a liquid system or for orchid species that do not require charcoal to neutralize phenolics produced by the plants.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 25.31
Other Notes Plant Tissue Culture Tested
UPC / SKU O139
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 825
B210 Boric Acid 3.1
C266 Calcium Chloride, Anhydrous 166
C350 Cobalt Chloride Hexahydrate 0.0125
C375 Cupric Sulfate, Pentahydrate 0.0125
E410 EDTA, Disodium Salt 37.3
F263 Ferrous Sulfate, Heptahydrate 27.85
M150 Magnesium Sulfate, Anhydrous 90.35
M250 Manganese Sulfate 8.45
P840 Potassium Iodide 0.415
P100 Potassium Nitrate 950
P846 Potassium Phosphate, Monobasic, Anhydrous 85
M651 Sodium Molybdate (VI), Dihydrate 0.125
Z101 Zinc Sulfate, Heptahydrate 5.3
I703 myo-Inositol 100
M825 MES, Free Acid 1000
N765 Nicotinic Acid 1.0
P775 Peptone from Meat 2000
P866 Pyridoxine HCl 1.0
S391 D-Sucrose 20,000
T390 Thiamine HCl 10

O139 Orchid Maintenance/Replate Medium Without Charcoal and Agar

Properties
Form: Powder
Appearance: White to Yellow powder
Application: Orchid Culture
Solubility: Water
Typical Working Concentration: 25.31 g/L
Storage Temp: 2-6 C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: pH = 4.75 - 5.7
Formula (mg/L)
Ammonium Nitrate 825
Boric Acid 3.1
Calcium Chloride, Anhydrous 166
Cobalt Chloride.6H2O 0.0125
Cupric Sulfate.5H2O 0.0125
Na2EDTA.2H2O 37.3
Ferrous Sulfate.7H2O 27.85
Magnesium Sulfate, Anhydrous 90.35
Manganese Sulfate.H2O 8.45
Molybdic Acid (Sodium Salt) .2H2O 0.125
Potassium Iodide 0.415
Potassium Nitrate 950
Potassium Phosphate, Monobasic 85
Zinc Sulfate.7H2O 5.3
MES (Free Acid) 1000
myo-Inositol 100
Nicotinic Acid (Free Acid) 1
Peptone from Meat 2000
Pyridoxine.HCl 1
Sucrose 20,000
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Many epiphytic orchid species
This medium is a modification of our original orchid replate medium (P668) except without charcoal (and agar). This medium permits the culture of orchids in a liquid system or for orchid species that do not require charcoal to neutralize phenolics produced by the plants.