Description: MPO, Mouse, ELISA kit

Myeloperoxidase (MPO) is a glycoprotein with a alpha2beta2 heteromultimer expressed in all cells of the myeloid linage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. MPO is rapidly released by activated polymorphonuclear neutrophils. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease and multiple sclerosis. Autoimmune antibodies to MPO are involved in Wegeners disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention.

Specifications

Catalog number HK210-02
Product type Assays
Quantity 2 x 96 det.
Standard range 1.6-100 ng/ml
Detection level 1.6 ng/ml
Working volume 100 l/well
Species Mouse
Cross reactivity Rat - Weak
Application The mouse MPO ELISA has been developed for the quantitative measurement of natural and recombinant mouse MPO in cell culture medium, plasma and tissue homogenates. In plasma samples MPO can be measured accurately if plasma samples are diluted at least 4 times. Most reliable results are obtained if Heparin plasma is used.
Disease Ageing Related Diseases, Cardiovascular Diseases, Pulmonary Diseases
Linearity The linearity of the assay was determined by serially diluting a serum sample containing 49.62 ng/ml mouse MPO. The diluted samples were measured in the assay. The line obtained a slope of 1.03 and a correlation coefficient of 0.998.
Storage and Stability Product should be stored at 4 C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
References 1. Vries de, B et al; Exogenous alpha-1-acid glycoprotein protects against renal ischemiareperfusion injury by inhibition of inflammation and apoptosis. Transplantation 2004, 78: 1116
2. Xu, H et al; A coagulation factor Vll deficiency protects against acute inflammatory responses in mice. J Pathol 2006, 210: 488
3. Porras, M et al; Correlation between cyclical epithelial barrier dysfunction and bacterial translocation in the relapses of intestinal inflammation. Inflamm Bowel Dis 2006, 12: 843