MTT Cell Proliferation Assay Kit provides a simple method for the determination of cell number using standard microplate absorbance readers. Determination of cell growth rates is widely used in the testing of drug action, cytotoxic agents and screening other biologically active compounds. Among a variety of non-radioactive cell proliferation assays, the MTT assay developed by Mossman (1) is still among one of the most versatile and popular assays.
The MTT assay is based on the cleavage of the yellow tetrazolium salt MTT to purple formazan crystal by metabolic active cells (2-4). The formazan is then solubilized, and the concentration determined by optical density at 570 nm. The result is a sensitive assay with a colorimetric signal proportional to the cell number. Biotium’s MTT Cell Proliferation Assay Kit provides ready-to-use reagents for performing 1000 individual assays using standard 96-well microplates. |