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Murashige & Skoog Basal Medium with Vitamins & 1.0 g/L MES
Product ID: M5531

Introduction

Description: Contains the macro- and micronutrients, and vitamins as described by Murashige & Skoog (1962). Modified to contain 1 g/L MES.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 5.43
Other Notes Plant Tissue Culture Tested
UPC / SKU M5531
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6
G503 Glycine 2.0
I703 myo-Inositol 100
M825 MES, Free Acid 1000
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
T390 Thiamine HCl 0.1

M5531 Murashige & Skoog (MS) Medium w/ MES

Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 5.43 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). Buffered with 1 g/L MES. pH = Approx. 4.0
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate  , Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
Glycine (Free Base) 2 myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
 Pyridoxine .HCl 0.5
Thiamine .HCl 0.1
MES 1000

Application Notes
Plant Tissue Culture Tested
Plant species: This medium is used for a numerous species.

References
Murashige, T and F Skoog. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.