Murashige & Skoog Modified Basal Medium with Sodium Phosphate and Ferric Sodium EDTA Product ID: P762
Introduction
Description: Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962) with the following exception: Without Potassium Phosphate Monobasic. Also contains (mg/mL): 300 Sodium Phosphate Monobasic, 150 Adenine Hemisulfate, 1000 Casein, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
5.69 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
M541 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
1650 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
332.2 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E676 |
Ferric Sodium EDTA |
36.7 |
| M150 |
Magnesium Sulfate, Anhydrous |
180.7 |
| M250 |
Manganese Sulfate |
16.9 |
| P840 |
Potassium Iodide |
0.83 |
| P100 |
Potassium Nitrate |
1900 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| S515 |
Sodium Phosphate, Monobasic |
300 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
| A545 |
Adenine Hemisulfate |
150 |
| C184 |
Casein, Enzymatic Hydrolysate |
1000 |
| G503 |
Glycine |
2.0 |
| I703 |
myo-Inositol |
100 |
| N765 |
Nicotinic Acid |
5.0 |
| P866 |
Pyridoxine HCl |
1.0 |
| T390 |
Thiamine HCl |
0.5 |
M541 Murashige & Skoog (MS)Modified Basal Medium
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow |
| Application: |
Plant Tissue Culture |
| Solubility: |
Soluble in Water |
| Typical Working Concentration: |
5.69 g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962) with the following exceptions: Potassium Phosphate Monobasic is omitted, Sodium Phosphate Monobasic is added, and Ferrous Sulfate and Disodium EDTA are replaced with Ferric Sodium EDTA. Approx. pH = 4.75 ± 0.5 |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
1650 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
332.2 |
| Cobalt Chloride•6H2O |
0.025 |
| Cupric Sulfate•5H2O |
0.025 |
| Ferric Sodium EDTA |
36.7 |
| Magnesium Sulfate, Anhydrous |
180.7 |
| Manganese Sulfate•H2O |
16.9 |
| Molybdic Acid (Sodium Salt)•2H2O |
0.25 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
1900 |
| Sodium Phosphate Monobasic |
300 |
| Zinc Sulfate•7H2O |
8.6 |
| Adenine Hemisulfate |
150 |
| Casein, Enzymatic Hydrolysate |
1000 |
| Glycine (Free Base) |
2 |
| myo-Inositol |
100 |
| Nicotinic Acid (Free Acid) |
5 |
| Pyridoxine•HCl |
1 |
| Thiamine•HCl |
0.5 |
Application Notes
Plant Tissue Culture Tested
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497
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