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Malmgren Modified Terrestrial Orchid Medium
Product ID: M534

Introduction

Description: Contains the macro- and micronutrients and organic constituents as described by Malmgren (1996). Without sucrose and agar.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 21.84
Other Notes Plant Tissue Culture Tested
UPC / SKU M534
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
C274 Calcium Phosphate, Tribasic 75
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 97.69
M250 Manganese Sulfate 1.54
P846 Potassium Phosphate, Monobasic, Anhydrous 75
C325 Activated Charcoal 1000
B140 D-Biotin 0.05
C184 Casein, Enzymatic Hydrolysate 400
F430 Folic Acid 0.5
G503 Glycine 2
I703 myo-Inositol 100
N765 Nicotinic Acid 5
P862 Pineapple Powder 20,000
P866 Pyridoxine HCl 5
T390 Thiamine HCl 10

M534 Malmgren Modified Terrestrial Orchid Medium Without Sucrose and Agar

Properties
Form: Powder
Appearance: Gray to Black
Application: Orchid Cultur
Solubility: Water
Typical Working Concentration: 21.84 g/L
Storage Temp: 2-6 C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro - and micronutrients and organic constituents as described by Malmgren (1996) . Does not contain Sucrose or Agar.
Formula (mg/L)
Calcium Phosphate, Tribasic 75
Na2 EDTA.2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 97.69
Manganese Sulfate.H2O 1.54
Potassium Phosphate, Monobasic 75
Activated Charcoal 1000
D-Biotin 0.05
Casein, Enzymatic Hydrolysate 400
Folic Acid 0.5
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 5
Pineapple Powder 20,000
Pyridoxine.HCl 5
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Terrestrial orchids including Paphiopedilum, Cypripedium, and Phragmipedium This medium is a good seed sowing medium for many terrestrial orchid species. Originally developed for Cypripedium, this medium is also used for germination of Phragmipedium and Paphiopedilum. Germination may be enhanced by includi ng 50 ml of Coconut Water (C195) per liter of medium.

References
Malmgren, S. 1996. In: C. Allen, Editor, North American Native Terrestrial Orchids. Propagation and Production Conf. Proc., pp 63 - 71.