King's B Medium Product ID: K5013
Introduction
Description: Non-selective general bacterial subculturing medium.
Often used for the detection & culture of pseudomonads such as Pseudomonas aeruginosa and Psuedomonas syringae by the addition of cephalexin & nystatin (see product info sheet for details) and for distinguishing fluorescent pseudomonads from nonfluorescent pseudomonads.
10 mL/L of Glycerol (G381) should be added to the medium before autoclaving.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
Room Temperature |
| Grams of powder to prepare 1 Liter |
37.23 |
| UPC / SKU |
K5013 |
| Synonyms |
KB Medium, KB Agar, King’s Agar B, King’s Base Medium; KBM; KB; King's B Agar; Pseudomonas Agar F; King B Medium; King B Agar |
| Storage Temp. |
Room Temperature |
| Tariff Code |
3821.00.0000 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| M150 |
Magnesium Sulfate, Anhydrous |
730 |
| P705 |
Potassium Phosphate, Dibasic |
1500 |
| A111 |
Agar |
15,000 |
| P775 |
Peptone from Meat |
20000 |
K5013 King’s B Medium
| Synonyms: |
KB Medium, KB Agar, King’s Agar B, King’s Base Medium; KBM; KB; King's B Agar; Pseudomonas Agar F |
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Off-White to Tan |
| Application: |
Phytopathology, Microbiology, Seed Testing, Plant Defense & Immunity |
| Solubility: |
Partially Soluble in Cold Water, Soluble in Boiling Water |
| Typical Working Concentration: |
37.23 g/L |
| Storage Temp: |
Room Temperature |
| Other Notes: |
Add 10-15 mL/L of Glycerol (Prod # G381) prior to autoclaving for carbon source.
Add 50 mg Cephalexin (Prod # C2112 or C1970) and 35 mg Nystatin (Prod # N581) per liter to select for Pseudomonads (Vogel & Bonner, 1956) |
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