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Isopropyl--D-thiogalactopyranoside (IPTG)
Product ID: I373

Introduction

Description: IPTG is a lactose-mimic which binds to the lac repressor to enhance expression of the lac Z gene
Synonym: Isopropyl--D-Thiogalactopyranoside

Solubility Water
Physical Form Solid
Storage Temp. 2 to 6 °C
UPC / SKU I373
CAS NUMBER 367-93-1
Formula Weight 238.3
Formula C9H18O5S
Synonyms Isopropyl-β-D-thiogalactopyranoside
Storage Temp. 2 to 6 C
Tariff Code 2932.99.9000
Risk Info (R) 36/37/38
Safety Info (S) 22-24/25-36
No information available

I373 Isopropyl-β-Dthiogalactopyranoside

Synonyms: IPTG
CAS: 367-93-1
Formula: C9H18O5S
Mol. Weight: 238.3
Properties
Form: Powder
Appearance: White Powder
Application: Molecular Biology
Solubility: Water @ 250 mg/mL
Typical Working Concentration:  
Storage Temp: 2 to 6°C
Storage Temp of Stock Solution: -20 to 0°C
Other Notes: Plant Tissue Culture Tested

Application Notes
Isopropyl-β-D-thiogalactopyranoside is often referred as IPTG. It acts as a substrate for β- glactosidase by inducing the expression of lac operon in Escherichia coli. IPTG binds to the repressor and modifies its form which then prevents the repression of lac gene coding by β- glactosidase. Additionally, IPTG is also a substrate and inducer for thiogalactoside transacetylase and penicillinase activity in bacteria, respectively

IPTG is often used in cloning procedures where induction of β-glactosidase activity is required. It is also widely used along side of X-Gal (Prod. No. X874) for blue-white colony screening of recombinant bacteria.

Blue/White Colony Screening:
1. Dissolve X-Gal (Prod. No. X874) in DMF (dimethylformamide) or DMSO (dimethyl sulfoxide) (Prod. No. D241) to make a 20 mg/ml solution. Wrap in aluminum foil to protect from light.
2. Prepare IPTG (Prod. No. I373) by dissolving 2 g in 8 ml of H2O, then adjust level to 10 ml and filter sterilize using a 0.22-micron filter. Dispense into 1mL aliquots.
3. Store both solutions at -20о C. Avoid freeze/thaw cycles as this can cause false results.
4. For use, spread 40 μL of stock X-Gal and 4 μL of IPTG onto surface of pre-made LB (prepared using LB Agar, Prod. No. L465) plate.