Hosta Initiation/Multiplication Medium Product ID: H435
Introduction
Description: Stage I/II medium. Modified Murashige & Skoog with (mg/L): 2.0 6-BA, 160 Adenine Hemisulfate, 500 Casein Hydrolysate, & 0.5 NAA.
Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and the vitamins described by Linsmaier & Skoog (1965). Also contains sucrose and agar.
| Solubility |
Water (Partially) |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
43.4 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
H435 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
1650 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
332.2 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
37.26 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
180.7 |
| M250 |
Manganese Sulfate |
16.9 |
| P840 |
Potassium Iodide |
0.83 |
| P100 |
Potassium Nitrate |
1900 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
300 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| S515 |
Sodium Phosphate, Monobasic |
170 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
| A545 |
Adenine Hemisulfate |
160 |
| A111 |
Agar |
8000 |
| B800 |
6-Benzylaminopurine (6-BA) |
2.0 |
| C184 |
Casein, Enzymatic Hydrolysate |
500 |
| G503 |
Glycine |
2.0 |
| I703 |
myo-Inositol |
100 |
| N600 |
a-Naphthaleneacetic Acid |
0.5 |
| S391 |
D-Sucrose |
30,000 |
| T390 |
Thiamine HCl |
0.4 |
H435 Hosta Initiation/ Multiplication Medium Stage I/II Medium
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow |
| Application: |
Plant Tissue Culture |
| Solubility: |
Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water |
| Typical Working Concentration: |
43.39 g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form |
| Other Notes: |
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains agar and sucrose. Plant Tissue Culture Tested. |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
1650 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
332.2 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Disodium EDTA .2H2O |
37.26 |
| Ferrous Sulfate.7H2O |
27.8 |
| Magnesium Sulfate, Anhydrous |
180.7 |
| Manganese Sulfate.H2O |
16.9 |
| Molybdic Acid (Sodium Salt). 2H2O |
0.25 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
1900 |
| Potassium Phosphate, Monobasic |
300 |
| Sodium Phosphate, Monobasic |
170 |
| Zinc Sulfate.7H2O |
8.6 |
| Adenine Hemisulfate |
160 |
| Agar |
8000 |
| 6-Benzylaminopurine (BA) |
2.0 |
| Casein, Enzymatic Hydrolysate |
500 |
| Glycine (Free Base) |
2 .0 |
| myo-Inositol |
100 |
| a-Naphthaleneacetic Acid |
0.5 |
| Sucrose |
30,000 |
| Thiamine.HCl |
0.4 |
Application Notes
Plant species: Hosta. Excellent stage I and II medium for Hosta cultures
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.
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