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DKW Basal Medium with 10 g/L Sucrose
Product ID: D191

Introduction

Description: Contains macro- and micronutrients as described by Driver & Kuniyuki (1984) and McGranahan et al. (1987). Contains 10 g/L sucrose. Without vitamins.
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 µM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 15.22
Other Notes Plant Tissue Culture Tested
UPC / SKU D191
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 1416
B210 Boric Acid 4.8
C266 Calcium Chloride, Anhydrous 112.5
C180 Calcium Nitrate 1367
C375 Cupric Sulfate, Pentahydrate 0.25
E410 EDTA, Disodium Salt 45.4
F263 Ferrous Sulfate, Heptahydrate 33.8
M150 Magnesium Sulfate, Anhydrous 361.49
M250 Manganese Sulfate 33.5
N458 Nickel Sulfate Hexahydrate 0.005
P846 Potassium Phosphate, Monobasic, Anhydrous 265
P854 Potassium Sulfate, Anhydrous 1559
M651 Sodium Molybdate (VI), Dihydrate 0.39
Z897 Zinc Nitrate, Hexahydrate 17

D191 DKW Basal Medium

Synonym: DKW Basal Salts w/ Sucrose;
Driver and Kuniyuki Walnut medium

Properties
Form: Powder
Appearance: White to Cream Colored Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 15.22 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Driver and Kuniyuki (1984) and McGranahan, et al. (1987). Does not contain vitamins, does contain 10 g/L sucrose. pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Nitrate  1416
Boric Acid 4.8
Calcium Chloride, Anhydrous 112.5
Calcium Nitrate 1367
 Cupric Sulfate·5H2O 0.25
Na2 EDTA·2H2O  45.4
 Ferrous Sulfate·7H2O 33.8
Magnesium Sulfate, Anhydrous 361.49
Manganese Sulfate·H2O  33.5
Molybdic Acid (Sodium Salt)·2H2O  0.39
Nickel Sulfate·6H2O 0.005
Potassium Phosphate, Monobasic 265
 Potassium Sulfate  1559
Zinc Nitrate·6H2O  17
 Sucrose  10,000

Application Notes
Plant species: Northern California Walnut (Juglans hindsii). This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 µM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse

References
Driver, J.A. and A.H. Kuniyuki. 1984. In vitro propagation of Paradox walnut rootstock. HortScience 19:507–509. McGranahan, GH, et al. 1987. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.