cDNA Synthesis Kit

List of Products..

M-MLV cDNA Synthesis Kit

M-MLV cDNA Synthesis Kit is designed for the highest efficiency conversion of RNA to cDNA. Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is a RNA directed DNA polymerase that can synthesize a complementary DNA (cDNA) strand from a primer hybridized to either RNA or single stranded DNA as a template. M-MLV Reverse Transcriptase is capable of synthesizing DNA longer than 5 kb from messenger RNA. RNase H activity of M-MLV Reverse Transcriptase is weaker than that of Avian Myeloblastosis Virus (AMV) Reverse Transcriptase. M-MLV Reverse Transcriptase does not possess 3'→5'exonuclease activity. A two-step RT-PCR format is useful for amplifying multiple targets from a single cDNA source, for maintaining archival cDNA, and for providing maximum flexibility in selecting a downstream qPCR reagent system. Read More..

SuperiorScript III cDNA Synthesis Kit

SuperiorScript III Reverse Transcriptase is genetically engineered version of M-MLV RT with reduced RNase H activity and increased thermostability, thus this enzyme can synthesize first-strand cDNA at elevated temperatures up to 55°C. SuperiorScript III Reverse Transcriptase shows improved theromostability and reduced RNase H activity than the competitor’s enzyme. This enzyme is capable of synthesizing first strand cDNA at 42°C~55°C with high specificity and yield. Synthesis of longer products (12 kb or more) are possible than the previous versions. Read More..

TOPscript cDNA Synthesis Kit

TOPscript™ cDNA Synthesis Kit is designed for the highest efficiency conversion of RNA to cDNA. TOPscript™ Reverse Transcriptase is genetically engineered version of M-MLV Reverse Transcriptase which is highly thermostable, thus can synthesize cDNA at elevated temperatures up to 60°C A two-step RT-PCR format is useful for amplifying multiple targets from a single cDNA source, for maintaining archival cDNA, and for providing maximum flexibility in selecting a downstream qPCR reagent system. The TOPscript™ cDNA synthesis Kit is formatted for high-yield production of cDNA up to 20 kb and each reaction accommodates a range of RNA amounts from fg to µg. Read More..