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Cephalexin
Product ID: C1970

Introduction

Description: Cephalexin is a beta-lactam antibiotic with activity against most gram-positive bacteria. Cephalexin acts by binding to specific penicillin-binding proteins (PBPs) inside the bacterial cell wall. It inhibits the last phase of cell wall synthesis, causing the eventual lysis of the bacterial cell.

This antibiotic is used with XTS Medium (X8454) and PhytoSelect Medium (P6800) for the isolation of: Xanthomonas translucens from barley and wheat seeds during seed testing, Xanthomonas campestris from Brassicas, and other plant pathogens.

See Product Information Sheet for details.
Synonym: Cefalexin

Solubility KOH
Grade USP
Use With P6800 - PhytoSelect Basal Medium; X8454 - XTS Medium
Physical Form Solid
Solutions Available C2112 (100 mg/mL)
Storage Temp. 2 to 6 °C
UPC / SKU C1970
CAS NUMBER 15686-71-2
Formula Weight 347.40 (anhydrous)
Formula C16H17N3O4S
Synonyms Cefalexin
Storage Temp. Room Temperature
Tariff Code 2941.90.3000
Risk Info (R) 42/43
Safety Info (S) 22-24/25
No information available

C1970 Cephalexin Monohydrate

Synonym: Cefalexin
CAS: 23325-78-2
Formula: C16H17N3O4S*H2O
Molecular Wt: 365.4
Properties
Form: Powder
Appearance: White to Off-White
Application: Antimicrobial
Solubility: KOH
Storage Temp: 2-6°C
Typical Working Concentration: Varies with application, should be determined by end user

Application Notes
Cephalexin is a beta-lactam antibiotic with activity against most gram-positive bacteria. Cephalexin acts by binding to specific penicillin-binding proteins (PBPs) inside the bacterial cell wall. It inhibits the last phase of cell wall synthesis, causing the eventual lysis of the bacterial cell.

Cephalexin can be used with PhytoSelect Basal Medium (Prod# P6800) in combination with other antimicrobials (see tabel below) to select for Xanthomonas campestris. Also recommended in the International Seed Testing Association’s (ISTA) Rules for Seed Testing in method 7-019 to test for Xanthomonas campestris pv. campestris on Brassica spp. (ISTA 2011).

Combine the following products to make 1L of SMART-Xca medium for Xanthomonas campestris (Kawanishi et al. 2011):

References
ISTA (2011) Annexe to Chapter 7: Seed Health Methods. Kawanishi T, T. Shiraishi, Y. Okano, K. Sugawara, M. Hashimoto, K Maejima, K Komatsu, S Kakizawa, Y Yamaji, H Hamamoto, K Oshima, S Namba (2011) New Detection Systems of Bacteria Using Highly Selective Media Designed by SMART: Selective Medium-Design Algorithm Restricted by Two Constraints. PLoS ONE Volume 6, Issue 1.

Prod # Name of Component: Amount to add to make 1L
P6800 PhytoSelect Basal Medium: 1L (24.927 g)
G503 Glycine: 1.0 g
*C1989 OR *C1796 Cycloheximide: 50 mg OR Cycoheximide Soln (100 mg/mL) – add 0.50 mL
*P777 Penicillin G: 10 mg
*C1970 OR *C2112 Cephalexin: 10 mg OR Cephalexin Soln (100 mg/mL) – add 0.10 mL