Introduction
Description: Bromothymol blue (BTB) is often used in electrophoresis to estimate the mobility of the electrophoretic front.
BTB is also a biological stain and pH indicator; yellow at pH 6.0, green at pH 7.0, and blue at pH 7.6.
Synonyms: BTB; 3',3''-dibromothymolsulfonephthalein sodium salt; Bromothymol Blue sodium salt; Bromothymol Sulfone Phthalein.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
Room Temperature |
| UPC / SKU |
C1933 |
| CAS NUMBER |
4193- 55-9 |
| Formula Weight |
960.95 |
| Formula |
C40H42N12O10S2 |
| Synonyms |
Calcofluor White ST |
| Storage Temp. |
Room Temperature |
No information available
C1933 Calcofluor White M2R
| Synonyms: |
Calcofluor White ST; Fluorescent Brightner 28 Disodium Salt; Cellufluor |
| CAS: |
4193-55-9 |
| Formula: |
C40H42N12O10S2Na2 |
| Mol. Weight: |
960.95 |
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Yellow to Tan |
| Application: |
Fluorescent Stain, Histology |
| Solubility: |
Water |
| Typical Working Concentration: |
Varies with application, should be determined by end user. |
| Storage Temp: |
Room Temperature |
Application Notes
Calcofluor White is a fluorescent stain that has been used to indicate cell wall biosynthesis for plant and fungal cells (Nagata & Takebe, 1970). Calcofluor White fluoresces when it hydrogen bonds with beta-1,4 and beta-1,3 glucosidic linkages, which are primary components of cellulose and chitin in cell walls. These types of fluorescent dyes are known as optical brightening agents because they absorb near-UV light (broad region lambda max = 315- 415 nm) and emit in the blue-green wavelength region (broad region lambda max = 430-550 nm). It can also be used for the staining of fungi and as a viability stain.
References
Nagata T. and Takebe I. (1970) Cell well regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta Vol. 92 pg. 301-308.
