Introduction
Description: Bromothymol blue (BTB) is often used in electrophoresis to estimate the mobility of the electrophoretic front. BTB is also a biological stain and pH indicator; yellow at pH 6.0, green at pH 7.0, and blue at pH 7.6.
Synonyms: BTB; 3',3''-dibromothymolsulfonephthalein sodium salt; Bromothymol Blue sodium salt; Bromothymol Sulfone Phthalein
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
Room Temperature |
| UPC / SKU |
B1714 |
| CAS NUMBER |
34722-90-2 |
| Formula Weight |
646.37 |
| Formula |
C27H27Br2O5SNa |
| Synonyms |
BTB; 3′,3′′-dibromothymolsulfonephthalein sodium salt; Bromthymol Blue sodium salt; Bromothymol Sulfone Phthalein |
| Storage Temp. |
Room Temperature |
| Tariff Code |
2914.70.4000 |
| Risk Info (R) |
38-41 |
| Safety Info (S) |
22-36/37/39 |
No information available
B1714 Bromothymol Blue, Sodium Salt
| Synonyms: |
Bromothymol Sulfone Phthalein, BTB, 3′,3″-Dibromothymolsulfonphthalein sodium salt |
| CAS: |
34722-90-2 |
| Formula: |
C27H27Br2O5SNa |
| Mol. Weight: |
646.37 |
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Orange to Black |
| Solubility: |
Soluble in Water |
| Application: |
Phytopathology, Seed Testing, Microbiology |
| Typical Working Concentration: |
Varies with application, should be determined by end user. |
| Storage Temp: |
Room Temperature |
Application Notes
Generally used as a biological stain and pH indicator. Bromothymol Blue (BTB) is yellow at a pH of 6.0 and gradually turns to blue as the pH rises to 7.6. It can be incorporated into a medium to observe color change, such as the inclusion of BTB in MSP agar (ISTA, 2011) or any other microbiology medium where a pH change can be indicative of bacterial/fungal growth.
References
ISTA (2011) Annexe to Chapter 7: Seed Health Methods. Kawanishi T, T. Shiraishi, Y. Okano, K. Sugawara, M. Hashimoto, K Maejima, K Komatsu, S Kakizawa, Y Yamaji, H Hamamoto, K Oshima, S Namba (2011) New Detection Systems of Bacteria Using Highly Selective Media Designed by SMART: Selective Medium-Design Algorithm Restricted by Two Constraints. PLoS ONE Volume 6, Issue 1.
