AuPreP™ Hotstart TaQ DNA Polymerase

An Outstanding Robust Polymerase for Hotstart PCR assays, highly suited for real time Assays with PCR products suitable for TA cloning


The product is highly suited for real time assays. The resulting PCR Products are suitable for TA cloning

Description- AuPreP Hotstart TaQ DNA Polymerase is a heat-activated thermostable DNA polymerase isolated from a novel organism. It provides improved specificity as compared to standard polymerases and can eliminate the presence of non-specifics, such as primer-dimers and mis-primed products. The enzyme is inactive at room temperature and therefore, prior to PCR cycling, requires activation by heat treatment for 10 minutes. Subsequently, the reaction can be handled according to the user’s existing protocols for thermostable DNA polymerases.


Reaction Conditions (for a 50μl reaction)

10x Buffer 5μl
50mM MgCl 2 1.5 – 4μl
100mM dNTP Mix (see below) 0.5 – 1μl
Template and Primers as required
AuPreP Hotstart DNA Polymerase 0.2 – 1μl
Water (ddH 2 O) up to 50μl
Activate: pre-heating step at 95°C for 10 minutes
Denature: 94-96°C
Elongate: 72°C (allowing 15-30 seconds/kb)

We recommend to use PCR DOCTOR for GC / AT / Dirty / Difficult Templates. Hence order it separately if needed. How to use the PCR

DOCTOR ? Compose the reaction mix, containing buffer, dNTPs, Mg 2+ , template DNA, primers, DNA polymerase. Add 2x PCR DOCTOR at the volume of half of the final volume of the reaction (e.g. 25ul per 50ul final volume, etc). Add ddH 2 O up to the final volume and mix with pipetting

This data is intended for use as a guide only; conditions will vary from reaction to reaction and may need optimization.

General Considerations:

The enzyme must be activated by heat treatment before PCR cycling. All reaction components (including AuPreP Hotstart DNA Polymerase) should be added to the reaction, and then pre-incubated at 95°C for 10 minutes. Subsequently, the reaction can be treated according to the user’s existing protocols for thermostable DNA polymerases. The ideal MgCl 2 concentration in the reaction is likely to be 1.5-2.5mM (final concentration),
but some optimization may be necessary to achieve the best possible results. For first tests, use no less than 1 unit of AuPreP Hotstart DNA Polymerase in a 50μl reaction.


250 Units   500 Units
AuPreP Hotstart DNA Polymerase 50μl 100μl
10x Buffer 1.2ml 2 x 1.2ml
50mM MgCl 2 Solution 1.2ml 1.2ml
Storage Conditions: @ -20°C for 1 year
Shipping Conditions: Dry Ice/ Blue Ice

Storage and Dilution Buffer:

20mM Tris-HCl, pH 7.5, 100mM NaCl, 0.1mM EDTA, 2mM DTT, 50% Glycerol, and stabilizers.

Associated Activities:

Endonuclease and exonuclease activities were not detectable after 4 hours of incubation of 1μg of pBR322 plasmid DNA and 0.5μg Hind III-digested lambda phage DNA at 72°C in the presence of 20u of AuPreP Hotstart DNA Polymerase.

Unit Definition:

One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C.
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AuPreP Hotstart TaQ DNA Polymerase (Robust Polymerase for Hotstart PCR assays)
AuPreP Super Fidelity TaQ DNA Polymerase (High fidelity Polymerase produces blunt ended amplicons upto 5Kb)
PCR Doctor - (PCR enhancement additive used with AuPreP Hotstart Taq or AuPreP Super Fidelity Taq
especially designed for GC/AT/Dirty/Difficult Templates
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Novascript III single step RT-PCR System (1step RT-PCR system using Novascript & AuPreP Hotstart DNA Polymerase)