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PhytoTechnology Laboratories

Agar, Micropropagation Grade
Product ID: A111

Introduction

Description: Agar, Plant TC Micropropagation Grade from Gracilaria sp.This is a higher gel-strength tissue-culture grade agar over A296.

Solubility Boiling Water
Physical Form Solid
Storage Temp. Room Temperature
Other Notes Plant Tissue Culture Tested
UPC / SKU A111
CAS NUMBER 9002-18-0
Synonyms Source: Gracilaria sp
Molecular Form test
Hydration test
Gelling Strength > 900 g/cm2
Storage Temp. Room Temperature
Tariff Code 1302.31.0000
Risk Info (R) 36/37/38
Safety Info (S) 36
No information available

A111 Agar, Plant TC Micropropagation Grade - Gracilaria

Synonyms: Agar-Agar, Gum Agar
CAS: 9002-18-0
Properties
Form: Powder
Appearance: Off-white to Cream
Application: Gelling Agent
Solubility: Partially Soluble in Cold Water; Soluble in Boiling Water
Typical Working Concentration: 6 to 10 grams per liter
Storage Temp: Room Temperature
Other Notes: Gel Strength: ≥900 g/cm2 Ash Content: <6% Plant Tissue Culture Tested

Application Notes
Agar is produced from a family of red seaweeds (Rhodophycae) primarily from two genera, Gelidium and Gracilaria. It is a mixture of agarose and agaropectin. Product A111 is produced from Gracilaria which has lower gel strength than those from Gelidium. However, in the 1950’s it was discovered that pre-treatment of the seaweed with alkali before extraction lowered the yield but gave an agar with higher gel strength.2

Agar is by far the most common gelling agent used in plant tissue culture. It is used at a wide range of concentrations from 6 g/L for a slightly firm gel to 10 g/L for a brick-like gel; however, 6-8 g/L is the more commonly used range.

PhytoTechnology Laboratories® also carries agar produced from Gelidium, Product No. A296, as well as high purity grade agar, Product No. A175.

Please Note: While PhytoTechnology Laboratories® tests each lot of this product with two or more plant cell/ tissue culture lines, it is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.

References
1.Merck 13, 184
2.McHugh, D.J. (2003). A guide to the seaweed industry. FAO Fisheries Technical Paper 441, Food and Agriculture Organization of the United Nations, Rome