WHY
RECOMBIVIRUS TECHNOLOGY IS MANY GENERATIONS AHEAD?
Animals, just like
humans, are susceptible to various bacterial and viral infections. Animals and
animal-derived cell lines are used widely in biomedical research. Laboratory
animal infections may compromise the health of the animals and ultimately the
research data derived from them. Therefore, it is critical to monitor the health
of laboratory animals and screen the presence of the animal viruses in cell
lines. A variety of Immunoassays (ELISA, IFA, HAI, Western blot) have been
developed to detect the presence of bacterial or virus specific antigen or
antibodies to assess animal health. ELISA offers many advantages over the other
immunoassay due to simple methodology, ability of handle large number of samples
in short time, relative low cost, and sensitivity of the technique.
Currently available
ELISA kits for the animal health screening employ viral extracts
prepared from virus grown in a variety of animal (Vero) and human (HEK) host
cell lines. These host cell lines are maintained in bovine or horse
serum. Therefore, the viral extracts used as antigen not only contain
majority of proteins derived from host cells but also residual serum protein of
bovine or horse origins. Therefore, it is necessaryto test samples on
viral antigen extracts and control cell extracts. It requires more
samples, more work, and sill yield low viral antibody detection.
Viral extracts are typically inactivated but there is always a
possibility of some residual live virus present that is capable of infecting
animals or cell lines. Due to this inherent design, viral extract dependent
ELISA suffers from many disadvantages such as-Low sensitivity, low
specificity, poor reproducibility and even detection of non-viral protein
antibodies. In addition, there is always a possibility of introducing
these viruses into animals, cell lines, and laboratory personnel.
Major antigenic proteins
of most animal viruses have been identified. With the advancement of recombinant
DNA technology, these viral proteins if expressed in E.coli, yeast or
baculovirus and purified provide the suitable answer to comer over the
shortfalls of conventional Elisa. Recombinant viral antigenic proteins
(envelop, nucleoprotein or membrane proteins) have been shown to be antigenic
and react with antibodies produced by the whole virus or during natural
infections. There is more consistency in the production and usage of recombinant
protein-based ELISA kits than the crude viral antigens. We are the first company
to commercially develop New generation Elisa Kits.
The RecombiVirusT
ELISA kits use the highly purified recombinant, antigenic, virus proteins
for the detection antibodies to various animal viruses. The New Generation Kits
for animal health screening havethus alleviated the inherent issues and problems
associated with the ELISA kits that use crude viral extracts. RecombiVirus T
ELISA kits offer not only better sensitivity, high specificity, reproducibility,
but also eliminated the possibility of virus contamination.
To learn more on
our technology and product details pl visit:
Why
Choose RecombiVirus Technology?
RecombiVirus
ELISAs are NEW Generation immunoassay using highly purified, recombinant
and antigenic proteins of animal viruses. These kits are superior for
enhanced sensitivity, specificity, stability, quantitative IgG/IgM
determination, smaller sample size, and safety. The kits do not need to
test samples with control host cell proteins; save precious samples and
reduce costs. All ELISA kits follow the same basic design, assay procedure
etc.
Learn more: http://atzlabs.com/pdf/ATZ_Labs_Animal_Viruses_Health_Monitoring_ELISA_Products.pdf
Parameter |
OLD GENERATION Crude viral protein Extracts based
Elisa |
RecombiVirusT ELISA using purified viral, antigenic,
recombinant proteins has clear ADVANTAGES |
Coating antigen |
Whole virus is propagated in host cells (mammalian) and crude
viral antigens (<1%) stock prepared |
Highly purified (>95%) recombinant (E. coli, baculovirus, or
Yeast) viral antigenic viral proteins. |
ELISA format |
Samples evaluated on host cell proteins alone as well as host
(cell + viral) proteins |
No need to test on control host cell proteins. Saves precious
samples and time as well. |
Sensitivity |
Low level of viral proteins coating compromise sensitivity and
increase background |
Purified viral proteins coated at high concentration increases
sensitivity and reduced background. |
Specificity |
Many viruses share several viral protein or have sequence
conservations leading to reduced specificity |
Coating antigen is carefully selected for its specificity for a
given virus |
Production issues |
Replication of live virus requires BSL-2/3 labs and the process
is labor intensive and viral yields are low. |
Recombinant proteins are expressed in relatively safe host such
as E. coli or yeast. No issues of virus contamination of animals or
humans. |
Reproducibility |
Due to inherent virus propagation issues reproducibility is
compromised |
Recombinant proteins purified in large
batches leading to more consistent and reliable test |
Antibody Quantitation Cut-Off
controls |
Provide only -ve. and +ve. controls. No Cut-off controls
are provided to help determine the status of-ve or +ve. Positive are
only qualitative. |
Kit contains -ve +ve control and a cut-off control.
Results can be expressed as -ve/+ve or antibody concn.
calculated from the standard curves. |
Overall assessment |
Whole viral antigen-based ELISA though offered some improvement
over HAL or IFA but recombinant protein bases assays are ultimate
& hence preferred. |
Recombinant viral protein based ELISA offer better format. Save
precious sample. Safe handling. High sensitivity and
specificity. |
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