Animals, just like humans, are susceptible to various bacterial and viral infections. Animals and animal-derived cell lines are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Therefore, it is critical to monitor the health of laboratory animals and screen the presence of the animal viruses in cell lines. A variety of Immunoassays (ELISA, IFA, HAI, Western blot) have been developed to detect the presence of bacterial or virus specific antigen or antibodies to assess animal health. ELISA offers many advantages over the other immunoassay due to simple methodology, ability of handle large number of samples in short time, relative low cost, and sensitivity of the technique.

Currently available ELISA kits for the animal health screening employ viral extracts prepared from virus grown in a variety of animal (Vero) and human (HEK) host cell lines. These host cell lines are maintained in bovine or horse serum. Therefore, the viral extracts used as antigen not only contain majority of proteins derived from host cells but also residual serum protein of bovine or horse origins. Therefore, it is necessaryto test samples on viral antigen extracts and control cell extracts. It requires more samples, more work, and sill yield low viral antibody detection. Viral extracts are typically inactivated but there is always a possibility of some residual live virus present that is capable of infecting animals or cell lines. Due to this inherent design, viral extract dependent ELISA suffers from many disadvantages such as-Low sensitivity, low specificity, poor reproducibility and even detection of non-viral protein antibodies. In addition, there is always a possibility of introducing these viruses into animals, cell lines, and laboratory personnel.

Major antigenic proteins of most animal viruses have been identified. With the advancement of recombinant DNA technology, these viral proteins if expressed in E.coli, yeast or baculovirus and purified provide the suitable answer to comer over the shortfalls of conventional Elisa. Recombinant viral antigenic proteins (envelop, nucleoprotein or membrane proteins) have been shown to be antigenic and react with antibodies produced by the whole virus or during natural infections. There is more consistency in the production and usage of recombinant protein-based ELISA kits than the crude viral antigens. We are the first company to commercially develop New generation Elisa Kits.

The RecombiVirusT ELISA kits use the highly purified recombinant, antigenic, virus proteins for the detection antibodies to various animal viruses. The New Generation Kits for animal health screening havethus alleviated the inherent issues and problems associated with the ELISA kits that use crude viral extracts. RecombiVirus T ELISA kits offer not only better sensitivity, high specificity, reproducibility, but also eliminated the possibility of virus contamination.

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Why Choose RecombiVirus Technology?

RecombiVirus ELISAs are NEW Generation immunoassay using highly purified, recombinant and antigenic proteins of animal viruses. These kits are superior for enhanced sensitivity, specificity, stability, quantitative IgG/IgM determination, smaller sample size, and safety. The kits do not need to test samples with control host cell proteins; save precious samples and reduce costs. All ELISA kits follow the same basic design, assay procedure etc.

Learn more: http://atzlabs.com/pdf/ATZ_Labs_Animal_Viruses_Health_Monitoring_ELISA_Products.pdf


OLD GENERATION Crude viral protein Extracts based Elisa

RecombiVirusT ELISA using purified viral, antigenic, recombinant proteins has clear  ADVANTAGES

Coating antigen

Whole virus is propagated in host cells (mammalian) and crude viral antigens (<1%) stock prepared

Highly purified (>95%) recombinant (E. coli, baculovirus, or Yeast) viral antigenic viral proteins.

ELISA format

Samples evaluated on host cell proteins alone as well as host (cell + viral) proteins

No need to test on control host cell proteins. Saves precious samples and time as well.


Low level of viral proteins coating compromise sensitivity and increase background

Purified viral proteins coated at high concentration increases sensitivity and reduced background.


Many viruses share several viral protein or have sequence conservations leading to reduced specificity

Coating antigen is carefully selected for its specificity for a given virus

Production issues

Replication of live virus requires BSL-2/3 labs and the process is labor intensive and viral yields are low.

Recombinant proteins are expressed in relatively safe host such as E. coli or yeast. No issues of virus contamination of animals or humans.


Due to inherent virus propagation issues reproducibility is compromised

Recombinant proteins purified in large

batches leading to more consistent and reliable test

Antibody Quantitation Cut-Off controls

Provide only -ve. and +ve.  controls. No Cut-off controls are provided to help determine the status of-ve or +ve. Positive are only qualitative.

Kit contains -ve   +ve control and a cut-off control. Results can be expressed as  -ve/+ve or antibody concn. calculated from the standard curves.

Overall assessment

Whole viral antigen-based ELISA though offered some improvement over HAL or IFA but recombinant protein bases assays are ultimate & hence preferred.

Recombinant viral protein based ELISA offer better format. Save precious sample. Safe handling. High sensitivity and specificity.