M-MLV cDNA Synthesis Kit

M-MLV cDNA Synthesis Kit is designed for the highest efficiency conversion of RNA to cDNA. Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is a RNA directed DNA polymerase that can synthesize a complementary DNA (cDNA) strand from a primer hybridized to either RNA or single stranded DNA as a template. M-MLV Reverse Transcriptase is capable of synthesizing DNA longer than 5 kb from messenger RNA. RNase H activity of M-MLV Reverse Transcriptase is weaker than that of Avian Myeloblastosis Virus (AMV) Reverse Transcriptase. M-MLV Reverse Transcriptase does not possess 3'→5'exonuclease activity. A two-step RT-PCR format is useful for amplifying multiple targets from a single cDNA source, for maintaining archival cDNA, and for providing maximum flexibility in selecting a downstream qPCR reagent system.

Related paper: