PCR Polymerases

Characteristics of Enzynomics PCR polymerases

 

nTaq

nTaq-HOT

nTaq-multi HOT

nTaq-Tenuto

nPfu-Forte

nPfu-Special

Length of amplified DNA

<5 kb

<5 kb

<5 kb

<15 kb

<15 kb

<20 kb

Yield

+

+

+

+++

++

+++

5'->3' Exonuclease

+a

+a

+a

+a

-

-

3'->5' Exonuclease

-

-

-

+b

++

++

Error rate (10-6)

24

24

24

3.0

0.28

0.11

Ends of amplified DNA

A tail

A tail

A tail

A tail

Blunt end

Blunt end

a 5’ to 3’ exonuclease activity is reduced by mutation.

b 3’ to 5’ proofreading activity of Pfu polymerase is present.

List of Products..

Standard PCR: nTaq

nTaq has greatly reduced activity of 5'→3' exonuclease activity. As a result, formation of erroneously amplified products caused by the 5'→3' exonuclease activity of wild type polymerase is effectively prevented

HOT-Start PCR: nTaq-HOT

nTaq -HOT is a hot start PCR polymerase which remains inactive at temperatures lower than 75°C as a result of the chemical modifications. Therefore, a heat activation step is required to activate

High Fidelity PCR: nPfu-Forte

nPfu-Forte displays higher fidelity than nTaq due to its 3'→5' proofreading exonuclease activity. thus, High-fidelity DNA polymerization is obtained with nPfu-Forte, allowing amplification of DNA up to 10 kb long.

High Performance PCR: nTaq-Tenuto

nTaq-Tenuto is nTaq supplemented with 3'→5’ proofreading activity and a PCR enhancing factor for improved efficiency and fidelity. nTaq-Tenuto can be used to amplify DNA longer than 10 kb, which is difficult with common Taq polymerases alone. Thus, this product is improved in both fidelity (> 2 fold) of PCR products and amplification efficiency of longer PCR products.

Multiplex PCR: nTaq-multi HOT

nTaq-multi HOT is a hot start PCR polymerase which remains inactive at temperatures lower than 75°C Therefore, a heat activation step is required to activate nTaq-multi HOT. nTaq-multi HOT produces up to 20 different amplified products with a single tube reaction. This product can be used in general multiplex PCR and genotyping experiment related to genetic diagnostics.

Clean PCR: nTaq-Pure

nTaq-Pure is a highly purified polymerase especially for PCR reactions where freedom from bacterial genomic DNA is essential. nTaq-Pure was purified by state of the art technology for minimizing the E. coli genomic DNA contamination.

Prevent carryover contamination PCR

Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA. Thus UDG plus PCR polymerase eliminate uracil-product amplicon carry-over.

Others

DNA Polymerases RNA Polymerases